Vol. 1, Issue 1, Part A (2019)

Cell or tissue culture relies on the fact that many cells in plant, animal and human have the ability to regenerate a whole organ of plant, animal or human. Single cells, plant cells without cell walls pieces of leaves, or root cell, embryonic cell, muscle tissue can often be used to generate a new organ, tissue on culture media giving the required growth factors like nutrients and hormones. Significant growth factors like moisture, light, oxygen, temperature, nutrition, culture media, hormones and growth regulators, sources of sample, sterile environment, incubator, cell density in 3D culture and microfluidic factors during cell or tissue culture method were noted well.