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International Journal of Clinical Biology and Biochemistry

Vol. 5, Issue 2, Part A (2023)

Investigation of β-lactamase-producing Escherichia coli from cancer patients

Author(s):

Aseel Ali Albadery, Amani Abd-Al-Ridha Al-Abdullah and Saad Shakir Mahdi Al-Amara

Abstract:

Background: E. coli is nosocomial bacteria which leading infections of the urinary tract in the patients with cancer. The emergence of β-lactam-resistant strains is a major source of concern in UTI treatment. resistant to β-lactam E. coli is becoming more of an issue everywhere, including in Iraq. The study estimate β -lactam-resistant E. coli prevalence in Basrah province/ Iraq.
Methods: The isolated uropathogens were detected by Vitek®2 technique, and the E. coli sensitivity to antibiotics was assessed. Additionally, E. coli were examined using the DAM and DDST techniques, and polymerase chain reaction (PCR) was used to find the main β -Lactamase genes blaTEM, blaOXA, blaCTX-M, and blaSHV.
Results: The urine samples were collected from patients with cancer (120) from Basrah center of the Oncology Al-Sader Teaching Hospital, who were suspected with UTIs. Biochemical tests used to identify bacterial growth have revealed a variety of bacterial species, with E. coli isolates accounting for the majority of cases (53.6%) and the other types (19.34%), respectively. The findings of this investigation revealed that among (n=22) E. coli isolates, the12 (54.55%) of the isolates produced extended spectrum-lactamases (ESBLs) with positive results. While employing the double-disc approximation method, the results for the 10 (45.45%) isolates showed no evidence of ESBL production. Polymerase chain reaction and double-disc synergy were used to detect the TEM, CTX-M, SHV, and OXA genes encoding ESBL. The percentage of genes found in the isolates under study were (100%), (31.8%), (100%), and (100%), respectively 
Conclusions: There is a spread of multiple types of ESBLs E. coli from UTIs cancer patients in Basra hospitals, and the isolated E. coli have a high ability to produce ESBLs against the third generation of cephalosporins and monobactam antibiotics.
 

Pages: 26-30  |  234 Views  56 Downloads

How to cite this article:
Aseel Ali Albadery, Amani Abd-Al-Ridha Al-Abdullah and Saad Shakir Mahdi Al-Amara. Investigation of β-lactamase-producing Escherichia coli from cancer patients. Int. J. Clin. Biol. Biochem. 2023;5(2):26-30. DOI: 10.33545/26646188.2023.v5.i2a.46
International Journal of Clinical Biology and Biochemistry

International Journal of Clinical Biology and Biochemistry

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